E2F1 anticorps (acLys120, acLys125)

N° du produit ABIN1107042

Cet anticorps anti-E2F1 est un anticorps Lapin Polyclonal détectant E2F1 dans WB, IHC (fro) et EIA. Adapté pour Humain et Souris.

Aperçu rapide pour E2F1 anticorps (acLys120, acLys125) (ABIN1107042)

Antigène: E2F1 (E2F Transcription Factor 1 (E2F1))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp E2F1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Détail du produit anti-E2F1 anticorps

Épitope: acLys120, acLys125

Specificité: Reacts with Human 47 kDa E2F-1 acK120 and acK125.

Réactivité croisée (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein A

Immunogène: Synthetic peptide derived from internal domain of Human E2F-1 protein containing acetyl lysine 120 and 125

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in distilled water.

Concentration: 1.0mg/mL

Buffer: 0.1 M Tris, 0.1 M Glycine, 2 % Sucrose, None

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Prior to reconstitution store the antibody at-20 °C. Store reconstituted antibody at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur E2F1

Antigène: E2F1 (E2F Transcription Factor 1 (E2F1))

Autre désignation: E2F1

Sujet: E2F-1 (also known as transcription factor E2F-1, Retinoblastoma binding protein 3, RBBP-3, PRB-binding protein E2F-1, PBR3, Retinoblastoma-associated protein 1 and RBAP-1) is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several domains con-served through evolution that are found in most members of the family. These domains include a DNA binding domain, a dimerization domain that determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein as well as E2F2 and E2F3 have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner and mediates both cell proliferation and p53-dependent/independent apoptosis. Increased nuclear expression of this protein has been reported in a variety of cancers.Synonyms: E2F transcription factor 1, E2F-1, PBR3, PRB-binding protein E2F-1, RBBP3, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3, Transcription factor E2F1

ID gène: 1869

NCBI Accession: NP_005216

UniProt: Q01094

Pathways: Signalisation p53, Cycle Cellulaire, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy